Allevi Bioink Guide
Want some guidance on what Allevi reagents to use with your Allevi bioprinter? Check out the information below to learn more about available reagents to purchase for use with your Allevi. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with an Allevi bioprinter. Still have more questions after reading this guide? Contact us at [email protected]
Materials used for bioprinting present a diverse selection, from hard acellular thermoplastics to cell suspensions completely void of extra material. These materials can be split into three main categories: Matrix, sacrificial and support. The goal of all of these materials is to fabricate viable constructs with high physiological relevance.
Cell Encapsulating Matrix Bioinks for Soft or Hard Tissue Applications
The cell-encapsulating materials below are compatible with a variety of cell types for many diverse applications in bioprinting. Use them on their own or combine with support or sacrificial materials for even more possibilities.
|Reagent||Application||Source||Resolution (mm)||Sacrificial Support?||Curing Reagent||More Info|
|Alginate||Soft Tissue||Natural||0.15 +/- 0.03||FRESH||Calcium Chloride
|Collagen||Soft Tissue||Natural||0.32 +/- 0.03||FRESH||None|
|Gelatin Methacrylate||Soft Tissue,
|Natural||0.40 +/- 0.03||None||LAP (BioKey)|
Support Bioinks for Hard Tissue Applications
Support bioinks are often used for connective or hard tissue applications, such as in the fabrication of cartilage, bone or muscle tissues, to name a few.
|Bioink||Melt Temperature (C)||Resolution (mm)||Documentation|
|Hyperelastic Bone||Room Temp||0.30 - 0.40|
|Polycaprolactone (PCL)||60||0.16 +/- 0.04|
|Poly (lactic-co-glycolic acid)
|72-77||0.15 +/- 0.01|
Sacrificial Bioinks for Temporary Support and Vasculature Channel Applications
These materials can be combined with matrix inks to fabricate vascular geometries within a structure, and are great for modeling vasculature as well as for microphysiological systems and organ-on-a-chip fabrication.
|Reagent||Method for Removal||Matrix Bioink Compatibility||Documentation|