Allevi Bioink Guide


Want some guidance on what Allevi reagents to use with your Allevi bioprinter? Check out the information below to learn more about available reagents to purchase for use with your Allevi. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with an Allevi bioprinter.  Still have more questions after reading this guide? Contact us at [email protected]com.

The main bioink categories: matrix, sacrificial and support. For a comprehensive review of all bioinks for extrusion bioprinting,    check out this paper!

The main bioink categories: matrix, sacrificial and support. For a comprehensive review of all bioinks for extrusion bioprinting, check out this paper!

Bioink Categories

Materials used for bioprinting present a diverse selection, from hard acellular thermoplastics to cell suspensions completely void of extra material.  These materials can be split into three main categories: Matrix, sacrificial and support. The goal of all of these materials is to fabricate viable constructs with high physiological relevance.

Cell Encapsulating Matrix Bioinks for Soft or Hard Tissue Applications

The cell-encapsulating materials below are compatible with a variety of cell types for many diverse applications in bioprinting. Use them on their own or combine with support or sacrificial materials for even more possibilities.

Reagent Application Source Resolution (mm) Sacrificial Support? Curing Reagent
Alginate Soft Tissue Natural 0.15 +/- 0.03 FRESH Calcium Chloride
Collagen Soft Tissue Natural 0.32 +/- 0.03 FRESH None
Gelatin Methacrylate Soft Tissue,
Hard Tissue
Natural 0.40 +/- 0.03 None LAP (BioKey)
PhotoHA Soft Tissue Natural 0.50 +/- 0.03 None LAP (BioKey)
Table 1: Matrix reagents, the cell-encapsulating materials in bioprinting, are the key player in the biofabrication process (Learn more about matrix bioinks here). Most matrix bioinks currently developed offer strengths and weaknesses, often meeting some but not all requirements necessary for bioprinting. Reagents in other categories can help combat these weaknesses.

Support Bioinks for Hard Tissue Applications

Support bioinks are often used for connective or hard tissue applications, such as in the fabrication of cartilage, bone or muscle tissues, to name a few. 

Bioink Melt Temperature (C) Resolution (mm) Documentation
Hyperelastic Bone Room Temp 0.30 - 0.40
Polycaprolactone (PCL) 60 0.16 +/- 0.04
Poly (lactic-co-glycolic acid)
72-77 0.15 +/- 0.01
Table 3: Support bioinks play a more permanent role in 3D printed constructs and are often used to adjust construct mechanical properties.

Sacrificial Bioinks for Temporary Support and Vasculature Channel Applications

These materials can be combined with matrix inks to fabricate vascular geometries within a structure, and are great for modeling vasculature as well as for microphysiological systems and organ-on-a-chip fabrication.

Reagent Method for Removal Matrix Bioink Compatibility
Pluronic F127 Cool GelMA
FRESH Heat Collagen
Carbohydrate Glass Heat PDMS
Table 2: Sacrificial bioinks are useful for materials with poor shape fidelity, as well as to create complex negative geometries such as vasculature networks, within a structure. Sacrificial materials, which can be washed away after printing, provide temporary support during the fabrication process.