Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in hard tissue engineering[1,2].
Storage and Handling
PLGA should be stored at – 20ºC in an airtight container.
You Will Need
- Petri dish or well plate for printing
- suggested: painter’s tape for improved first layer adhesion
- Stainless Steel Allevi Syring e
Instructions for Use
*If printing with cell-laden matrix bioinks, all steps must be completed in a sterile environment. Be sure to sterilize PLGA overnight prior to use with cells.
- If using with cells, first sterilize PLGA.
- Measure the desired amount of PLGA for printing and load into metal syringe.
- Cap the metal syringe with a metal needle.
- Load syringe into the first extruder in the Allevi printer. Connect to your Allevi printer through your computer.
- Set the heat for the first extruder to suggested print temperature and allow time for PLGA to melt.
- Optional: To help with adhesion of the material, painter’s tape is suggested as a printing surface.
|Speed (mm/s)||Layer Height (mm)||Nozzle Diam (mm)||Gauge||Pressure (psi)||Print Temp (C)|
Try it out!
Try a practice print by following the instructions for Your First Bioprint using the print parameters and loading instructions for PLGA.
Print speed, nozzle diameter and pressure may all be adjusted to better fit a user’s needs. For help adjusting print parameters please contact [email protected]
A fill volume change of more than 2 ml may affect pressure settings.
A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution
 Pati, Falguni et al, “Ornamenting 3D Printed Scaffolds with Cell-laid Extracellular Matrix for Bone Tissue Regeneration” Biomaterials, 2015(37), pp. 230 – 241.