PLGA Bioprinting

 
 
 

Overview

Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in hard tissue engineering[1,2].

Storage and Handling

PLGA should be stored at – 20ºC in an airtight container.

You Will Need

  • PLGA

  • Needles
  • Petri dish or well plate for printing
  • suggested: painter’s tape for improved first layer adhesion
  • Stainless Steel Allevi Syring e

Instructions for Use

*If printing with cell-laden matrix bioinks, all steps must be completed in a sterile environment. Be sure to sterilize PLGA overnight prior to use with cells.

  1. If using with cells, first sterilize PLGA.
  2. Measure the desired amount of PLGA for printing and load into metal syringe.
  3. Cap the metal syringe with a metal needle.
  4. Load syringe into the first extruder in the Allevi printer. Connect to your Allevi printer through your computer.
  5. Set the heat for the first extruder to suggested print temperature and allow time for PLGA to melt.
  6. Optional: To help with adhesion of the material, painter’s tape is suggested as a printing surface.

Print Settings

*Critical step: Your needle can have significant effects on print settings. Be sure to use the suggested needle type or re-characteriz print parameters.
Speed (mm/s) Layer Height (mm) Nozzle Diam (mm) Gauge Pressure (psi) Print Temp (C)
1.0 0.2 0.15 30 Tapered 100 95

Try it out!

 
  Figure 1: PLGA lattice (left), cylinder (middle) and line (right) structures printed with the Allevi 2.

Figure 1: PLGA lattice (left), cylinder (middle) and line (right) structures printed with the Allevi 2.

 

Try a practice print by following the instructions for Your First Bioprint using the print parameters and loading instructions for PLGA.

Notes

  1. Print speed, nozzle diameter and pressure may all be adjusted to better fit a user’s needs. For help adjusting print parameters please contact info@allevi3d.com.

  2. A fill volume change of more than 2 ml may affect pressure settings.

  3. A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution

References

[1] Pati, Falguni et al, “Ornamenting 3D Printed Scaffolds with Cell-laid Extracellular Matrix for Bone Tissue Regeneration” Biomaterials, 2015(37), pp. 230 – 241.