Glass Slide Treatment

 

Print surfaces in bioprinting can have a large impact on final results [1]. This glass slide treatment can be used for printing with GelMA or PEGDA to keep structures adhered to a specific surface, allowing for improved maneuvering and control over printed structures.

You Will Need:

Protocol:

  1. First, sonicate glass slides for 5 minutes in ethanol.

  2. Replace with new ethanol solution and sonicate again for another 5 minutes.

  3. Sonicate glass slides in deionized water.

  4. Under a chemical hood, prepare a solution of 5% TMSPMA (v/v) in toluene.

  5. Soak slides in prepared solution overnight at 60°C.

  6. Remove slides from TMSPMA solution and wash with two ethanol rinses and two deionized water rinses.

  7. Sterilize slides in ethanol before use with cells.

[1] Jia Min Lee & Wai Yee Yeong (2015) A preliminary model of time-pressure dispensing system for bioprinting based on printing and material parameters, Virtual and Physical Prototyping, 10:1, 3-8