FRESH Bioprinting Method
Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). The method utilizes a support hydrogel as a temporary, thermoreversible support that can be washed away after printing. The FRESH support reagent is comprised of processed gelatin microparticles with a Bingham plastic rheology. This biocompatible material offers temporary support of fragile materials used for bioprinting. This method is compatible with all Allevi bioprinters (check out the video!). You can buy reagents for FRESH printing method in our shop.
For collaborations or inquiries about the FRESH method, contact Adam Feinburg. For a protocol of the FRESH method with a Allevi 2, please contact [email protected]
Hinton, T. J. et al, “Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels,” Science Advances, vol. 1, no. 9, October 2015.