Gelatin is a sacrificial bioink that can be used for temporary support or to create channels, vessels, or vasculature for bioprinting purposes. Through a reversible thermal gelatin, gelatin remains solid at room temperature and can be melted away at 37°C. The recommended use for gelatin as a sacrificial material is as a FRESH support material. Below are results from printing gelatin individually.
Storage and Handling
Gelatin should be stored at room temperature in a dry environment.
You Will Need
In addition to gelatin, you will also need:
We suggest these tapered 25 gauge needles.
Petri dish or well plate for printing
Optional: transwell plates can help avoid sample loss or damage throughout experiment. Keep in mind though that transwell membranes may inhibit imaging processes.
We suggest these for gelatin solutions.
Gelatin was dissolved in deionized water at 60°C in formulations of 5% (w/v) or 10% (w/v), then loaded into a syringe to half the total syringe volume and allowed to cool to room temperature. Print files of lines (included below) were completed at varying speeds and pressures. Three prints were completed at each setting and analyzed with ImageJ software.
|SLICING PARAMETERS||PRINT PARAMETERS|
|Gelatin Concentration (%)||Layer Height (mm)||Speed (mm/s)||Pressure (PSI)||Temperature (°C)||Needle||Resolution (mm)|
|5||0.3||10||22||RT||1.25" tapered 25G||0.90 ± 0.10|
|5||0.3||12||15||RT||1.25" tapered 25G||0.59 ± 0.14|
|5||0.3||14||22||RT||1.25" tapered 25G||0.72 ± 0.10|
|10||0.3||14||63||RT||1.25" tapered 25G||0.43 ± 0.21|